文章基本信息

标题：Detection of Antibody and the Antigen Classes in Circulating Immune Complexes Fractionated with the Sucrose Density Gradient Centrifugation Using an F(ab′)2anti-C3 ELISA
作者：Makoto Muratsugu
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：2005
卷号：51
期号：5
页码：584-589
DOI：10.1248/jhs.51.584
出版社：The Pharmaceutical Society of Japan
摘要：Circulating immune complexes (CICs) in sera from patients with liver cirrhosis (LC), primary biliary cirrhosis (PBC), hepatitis B type (BCH) and hepatitis not including BCH (nonBCH) were analyzed using F(ab′)₂anti-C3 ELISA, since CICs' functions are intimately associated with the composition and their sizes. Positive percentages for IgG-, IgA- and IgM-CIC were significantly increased in LC, PBC, BCH and nonBCH as compared to healthy specimens. The combination of F(ab′)₂anti-C3 ELISA and sucrose density gradient fractionation allowed us to detect the classes of antibodies and hepatitis B surface antigens in the fractionated CICs from sera of patients with liver diseases. Properties of the fractionized CICs are discussed in the text.
关键词：immune complex;F(ab′)2anti-C3 ELISA;sucrose density gradient fractionation;hepatitis B surface antigen;immunoglobulin