摘要：To elucidate a physiological function of dried-bonito broth (DBB) on blood fluidity and oxidative stress, we performed a randomized double-blind placebo-controlled study in twenty-four healthy adult subjects. The subjects ingested DBB or a placebo for four weeks, and blood fluidity and oxidative stress were measured before and after ingestion. Blood fluidity was measured using a microchannel array flow analyzer by the passage time of 100 μl of heparinized whole blood through the microchannel array, while oxidative stress was evaluated as a level of derivative of reactive oxygen metabolites (d-ROMs) by a free radical analysis system (FRAS). DBB ingestion significantly shortened the blood passage time from 55.4±3.4 to 47.6±2.0 sec (mean±SEM, p <0.05), while no significant change was observed in the placebo group (52.4±3.4 to 51.4±2.6 sec, mean±SEM) indicating that DBB ameliorated blood fluidity. The level of d-ROMs, known as a biomarker of oxidative stress, significantly decreased after DBB ingestion from 337.2±18.5 to 316.5±12.9 Carrotelli units (Carr. U.) (mean±SEM, p <0.05), suggesting that DBB reduced oxidative stress. Among subjects with a d-ROMs score >320, regarded as being in a state of oxidative stress, changes in blood fluidity tended to correlate with changes in d-ROMs score (ρ=0.55, p =0.06), showing that blood fluidity may have improved in subjects whose oxidative stress was markedly decreased. These results also showed a possibility that DBB ingestion improved blood fluidity by decreasing oxidative stress. In previous studies, daily DBB ingestion improved various fatigue-related symptoms, so we investigated the effect of DBB on fatigue-related symptoms via a questionnaire survey in the present study. The result of this survey showed that symptoms of shoulder stiffness and visual fatigue were improved only in the DBB group ( p <0.05, p <0.1, respectively). Insufficient blood circulation is considered to lead to the development of shoulder stiffness, visual fatigue, and other fatigue-related symptoms. Based on these findings, we considered that dietary intake of DBB may improve blood fluidity by reducing oxidative stress and thus might protect against fatigue.
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