摘要：Paclitaxel-induced peripheral neuropathy is a condition of nerve degeneration induced bychemotherapy, which afflicts up to 70% of treated patients. Therapeutic interventions are unavailabledue to an incomplete understanding of the underlying mechanisms. We previously discoveredthat major physiological changes in the skin underlie paclitaxel-induced peripheral neuropathy inzebrafish and rodents. The precise molecular mechanisms are only incompletely understood. Forinstance, paclitaxel induces the upregulation of MMP-13, which, when inhibited, prevents axondegeneration. To better understand other gene regulatory changes induced by paclitaxel, we inducedperipheral neuropathy in mice following intraperitoneal injection either with vehicle or paclitaxelevery other day four times total. Skin and dorsal root ganglion neurons were collected based ondistinct behavioural responses categorised as “pain onset” (d4), “maximal pain” (d7), “beginning ofpain resolution” (d11), and “recovery phase” (d23) for comparative longitudinal RNA sequencing.The generated datasets validate previous discoveries and reveal additional gene expression changesthat warrant further validation with the goal to aid in the development of drugs that prevent orreverse paclitaxel-induced peripheral neuropathy.
