摘要:The sandeel species (Ammodytes spp.) occupy a critical ecological position in connecting lower trophic levels to higher ones. However, they are strongly affected by the marine environment and their catch rates are trending downward. In this study, the target strength (TS) of sandeel species was measured in free-swimming specimens using a split-beam quantitative echo sounder with 38 and 120 kHz frequencies in a physically controlled environment. Parameters a and b used in the estimated TSmean–fork length (FL: 13.5–21.5 cm) equation were 53.7 and −124.3 dB at 38 kHz, and 71.3 and −153.2 dB at 120 kHz, respectively. The TS of the sandeel species were not proportional to the square of the FL but increased relatively rapidly with increasing body size. In addition, the mean and standard deviation of the swimming angle for the sandeel species from the acoustic data at 120 kHz were −2.2° and 7.7°, respectively, and most were in the −30° to 30°, range. Furthermore, TS was stronger at 38 kHz than at 120 kHz for all FL classes. The use of such frequency characteristics could facilitate the discrimination of fish species in the field and the sustainable assessment of sandeel species stocks.