摘要：Turbulence is one of the ubiquitous aspects of aquatic systems and affects many physical and biological processes. Based on direct velocity measurements and a computational fluid dynamics (CFD) simulation, we characterized the distribution of the turbulent kinetic dissipations rates (ε) in an orbital shaker system within a range of rotation frequencies. CFD was able to estimate the ε distribution in containers accurately, which was confirmed by other two methods and was independent of velocity measurement. The results showed that ε was linearly correlated with the rotational frequencies. Despite the existence of gradients of ε and the fact that a mean circular horizontal flow was formed within the tank, the energy levels of the whole tank varied spatially within an order of magnitude and the ε distributions at different rotational frequencies were similar, suggesting that the ε distribution in the whole tank could be seen as quasi-homogeneous. To investigate the influence of turbulence on algae growth, culture experiments of a typical diatom—Skeletonema costatum were carried out under different turbulence conditions. Our results suggested turbulence mixing promoted nutrient uptake and growth of Skeletonema costatum, which could be attributed to the break of the diffusion-limited resource concentration boundary layer surrounding phytoplankton.
关键词：turbulence; orbital shaker; phytoplankton culture
