摘要：Tillage leads to rapid loss of soil nitrogen (N) over a short period of time in karst areas. N leaching is the primary pathway of soil N loss and therefore is key to understanding the mechanisms of N loss induced by tillage. However, the factors affecting N leaching under tillage are not fully understood. Effects of tillage at various frequencies on leached N were examined in a one-year in situ simulation experiment using five tillage treatments: no tillage (T0), semiannual tillage (T1), and tillage every four months (T2), two months (T3), and monthly (T4). Concentration and amount of leached N had peaks in dry–rewetting months. Tillage significantly increased total amounts of leached N during the one-year experiment, and the largest amount of leached N was under tillage at the highest frequency. The primary form of N in leachate was NO3¯ (88.49–91.11%), followed by DON (7.80–9.87%), and then NH4+ with the lowest amount (1.09–2.10%). Tillage increased the amount of leached NO3¯ and DON, but had no significant effect on leached NH4+. Additionally, the amount of leached N had significantly negative correlations with 5–8 mm soil aggregate, NO3¯, DON, and sand content, and positive correlations with 2–5 and 0.25–2 mm. Soil 5–8 mm aggregate and DON were the main factors explaining the variation in leached N according to the RDA analysis. Tillage increased the breakdown of large aggregates, appearing to have increased the mineralization of organic matter, which resulted in increased N leaching. Our results emphasize the importance of reducing or eliminating physical disturbance indued by tillage and maintenance of large soil aggregates for decreasing N leachate in lime soil of karst regions.
关键词：karst ecosystem; human disturbance; nitrate; leachate; aggregate
