摘要：SummaryThe availability of cost-effective, highly portable, and easy to use high-resolution live-cell imaging systems could present a significant technological break-through in challenging environments, such as high-level biosafety laboratories or sites where new viral outbreaks are suspected. We describe and demonstrate a cost-effective high-speed fluorescence microscope enabling the live tracking of virus particles across virological synapses that form between infected and uninfected T cells. The dynamics of HIV-1 proteins studied at the cellular level and the formation of virological synapses in living T cells reveals mechanisms by which cell-cell interactions facilitate infection between immune cells. Dual-color 3D fluorescence deconvolution microscopy of HIV-1 particles at frames rates of 100 frames per second allows us to follow the transfer of HIV-1 particles across the T cell virological synapse between living T cells. We also confirm the successful transfer of virus by imaging T cell samples fixed at specific time points during cell-cell virus transfer by super-resolution structured illumination microscopy.Graphical abstractDisplay OmittedHighlights•A cost-effective, high-speed 3D fluorescence microscope was developed•Three-dimensional fluorescence micrographs of immune cells were collected•Transfer of HIV-1 between infected and uninfected T cells was observed•Super-resolution SIM imaging revealed HIV-1 particles in immunological synapsesOptical imaging; Virology
