摘要：SummaryMushroom cultivation is a sustainable agricultural waste utilization method, but the lack of high-value utilization of the produced spent mushroom substrate (SMS) has hindered the development of mushroom cultivation-based circular agricultural systems. Conversion and utilization of SMS viaProtaetia brevitarsislarvae (PBL) have proven to be a high-value AASMS utilization strategy. However,Auricularia auriculaSMS (AASMS), which contains woodchips, is less palatable and digestible for PBL. To solve this problem, in this investigation, we screened out microflora (MF) for AASMS fermentation by comparing the fermentation performance as well as the effect on PBL feed intake, weight gain, and AASMS phytotoxic compound removal efficiency. In addition, by bacterial community analysis, the generaLuteimonas,Moheibacter, andPseudoxanthomonaswere predicted to be functional bacteria for AASMS fermentation and contribute to palatability and digestibility improvement.Graphical abstractDisplay OmittedHighlights•Larvae frass microflora can fermentAuricularia auriculaspent mushroom substrate•The fermentation can improve feed intake, weight gain, and phytotoxic removal efficiency•The genera Luteimonas, Moheibacter, and Pseudoxanthomonas were functional bacteriaNatural resources; Entomology; Food science
