期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2006
卷号：52
期号：1
页码：66-69
DOI：10.3177/jnsv.52.66
出版社：Center for Academic Publications Japan
摘要：We studied the effect of incubation temperature on the production of short-chain fatty acids (SCFA) by pig cecal bacteria in vitro in order to assess short-term influences of body temperature on bacterial metabolism in the large intestine. We employed a 200 mL scale continuous culture system using cecal bacteria from commercially slaughtered pigs as innoculum. The culture was maintained at 30, 37, 40 or 42°C and continuously diluted by continuous feeding of bicarbonate buffer (pH 7.4) added with lactose (10 g/L) and by simultaneous continuous efflux both at 4.17 mL/h. We monitored SCFA concentration of the culture for 12 h, which represents their production rate. Concentrations of SCFA increased during the first several hours and plateaued at around 11 h of incubation. Incubation temperature significantly affected mean concentrations from 1 to 12 h of acetic (40°C>42°C=37°C>30°C), propionic (40°C>42°C=30°C), n -butyric (42°C>37°C>30°C, 40°C>30°C) and n -valeric (42°C=40°C>37°C>30°C) acids, and total SCFA (40°C>42°C=37°C>30°C) ( p <0.05). These results indicate that both hyperthermia and hypothermia depress the microbial breakdown of carbohydrates.
