期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2006
卷号：52
期号：1
页码：79-81
DOI：10.3177/jnsv.52.79
出版社：Center for Academic Publications Japan
摘要：We developed a novel method that conveniently measures dietary choline-phospholipid content. Crude lipids extracted from dietary samples were reacted with phospholipase D from Streptomyces chromofuscus . The choline liberated from this reaction was then reacted with potassium triiodide, yielding choline periodide, which could be measured spectrophotometrically at 365 nm. This method proved to be more convenient than conventional assays, such as thin layer chromatography and high performance liquid chromatography. Our novel method is suitable for measuring many samples in single experiments.
关键词：choline;lecithin;dietary intake;phospholipase D
