期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2008
卷号：54
期号：4
页码：303-308
DOI：10.3177/jnsv.54.303
出版社：Center for Academic Publications Japan
摘要：Chromium (Cr) is an essential trace element and is important for normal carbohydrate metabolism, and its deficiency in animals can cause a diabetic-like state. Human and experimental animal studies suggest that urinary Cr excretion is increased in diabetic populations. To investigate whether hyperglycemia-induced elevation of urinary Cr excretion reduces tissue Cr storage conditions, we assessed total Cr balance and Cr distribution in streptozotocin (STZ)-induced diabetic mice. Male C57BL mice were randomly assigned to STZ or control groups and their urine was collected 7, 14, 21 and 28 d after STZ injection. An inductively coupled plasma mass spectrometry instrument equipped with a dynamic reaction cell was used for determination of Cr in urine, plasma and tissues samples. The urinary excretions of Cr were 15.4±3.0 and 356±62 ng/d, and the renal Cr concentrations were 0.85±0.12 and 0.17±0.03 ng/mg for the control and diabetes groups, respectively ( p <0.01), after 28 d. The Cr balance in STZ-treated mice was distinctly negative due to the increase in urinary Cr loss ( p <0.01). These results suggest that in mice, STZ induces a reduction in renal Cr concentration and total negative Cr balance caused by an increase in urinary Cr output.
