期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2009
卷号：55
期号：3
页码：292-295
DOI：10.3177/jnsv.55.292
出版社：Center for Academic Publications Japan
摘要：A method for the determination of γ-aminobutyric acid (GABA) in foodstuffs has been developed by combination of its dinitrophenylation and high-performance liquid chromatography (HPLC) using norleucine as an internal standard. GABA was converted to its stable derivative with 1-fluoro-2,4-dinitrobenzene and the derivative was extracted with ether. After evaporation of the extract, the residue was dissolved in 0.1 M NaOH and the solution was subjected to reversed-phase HPLC with an elution system of a linear gradient of methanol in 10 m M Tris-HCl buffer (pH 6.0) and a detection system monitoring the absorbance of the effluent at 400 nm. The present method was shown to be utilized as a satisfactory method for the determination of γ-aminobutyric acid in foodstuffs.
关键词：γ-aminobutyric acid;HPLC;DNP-derivative;determination of γ-aminobutyric acid
