标题：Changes in N-Acetylglutamate Are Involved in Regulating Urea Synthesis in Rats Given a Low Gluten Diet Supplemented with L-Lysine, L-Methinone and L-Threonine
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2009
卷号：55
期号：5
页码：417-422
DOI：10.3177/jnsv.55.417
出版社：Center for Academic Publications Japan
摘要：We have shown that urinary urea excretion decreased in rats fed a low gluten diet supplemented with dietary limiting amino acids. The purpose of present study was to determine whether the addition of dietary limiting amino acids to a low gluten diet affected the synthesis and degradation of N -acetylglutamate and regulated urea synthesis. Experiments were done on two groups of rats, given diets containing 10% gluten or 10% gluten+0.5% L -lysine, 0.2% L -threonine and 0.2% L -methionine for 10 d. The urinary excretion of urea, and the liver concentration of N -acetylglutamate, and the liver activity of N -acetylglutamate synthetase decreased with the addition of dietary L -lysine, L -threonine and L -methionine. N -Acetylglutamate concentration in the liver was closely correlated with the N -acetylglutamate synthetase activity in the liver and excretion of urea. The greater degradation of N -acetylglutamate was observed in the group fed the 10% gluten+ L -lysine, L -threonine and L -methionine. The hepatic concentration of glutamate and plasma concentration of arginine were not related to the N -acetylglutamate concentration in the liver. These results suggest that the addition of limiting amino acids to the low gluten diet controls the synthesis and degradation of N -acetylglutamate in the liver and lowers urea synthesis.
