标题：Suppressive Effect of an Isoflavone Fraction on Tumor Necrosis Factor-α-Induced Interleukin-8 Production in Human Intestinal Epithelial Caco-2 Cells
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2009
卷号：55
期号：5
页码：442-446
DOI：10.3177/jnsv.55.442
出版社：Center for Academic Publications Japan
摘要：This study demonstrates the effect of soybean components on the tumor necrosis factor-α (TNF-α)-induced production of interleukin-8 (IL-8), one of the major inflammatory chemokines, in intestinal epithelial-like Caco-2 cells. Among the soybean components, an isoflavone fraction (IFF) suppressed the TNF-α-induced IL-8 secretion by Caco-2 cells in a dose-dependent manner, whereas a soyasaponin fraction and soypeptide fraction had no significant effect on TNF-α-induced IL-8 secretion. The IL-8 secretion induced by hydrogen peroxide and by IL-1β was not suppressed by IFF, suggesting that the inhibitory effect of isoflavone was specific for the TNF-α-induced regulation of IL-8. The increased expression of IL-8 mRNA by TNF-α was almost completely suppressed by IFF. Furthermore, the transcriptional activity of the human IL-8 promoter was increased by the TNF-α treatment, and IFF significantly suppressed the IL-8 promoter activity. These results indicate that IFF suppressed TNF-α-induced IL-8 production at the transcriptional level in human intestinal Caco-2 cells, suggesting IFF of soybean as a promising food component for preventing intestinal inflammation such as inflammatory bowel disease.
