期刊名称:Journal of Nutritional Science and Vitaminology
印刷版ISSN:0301-4800
电子版ISSN:1881-7742
出版年度:2012
卷号:58
期号:1
页码:50-53
DOI:10.3177/jnsv.58.50
出版社:Center for Academic Publications Japan
摘要:Vitamin B12 content of an edible cyanobacterium, Nostochopsis sp. was determined to be 140.6±16.2 μg/100 g dry weight by a microbiological method. To evaluate whether the Nostochopsis cells contain vitamin B12 or inactive corrinoid compounds, corrinoid compounds were purified from the cells and then identified as pseudovitamin B12 (97.4±11.8 μg/100 g dry weight) and vitamin B12 (43.2±6.0 μg/100 g dry weight) on the basis of silica gel 60 TLC bioautograms and LC/ESI-MS/MS chromatograms. Vitamin B12 content was significantly increased in the Nostochopsis cells (254.8±17.6 μg/100 g dry weight) grown in the vitamin B12-supplemented medium.