Objectives: This was a randomized controlled pilot trial to evaluate the effectiveness of cupping therapy for neck pain in video display terminal (VDT) workers. Methods: Forty VDT workers with moderate to severe neck pain were recruited from May, 2011 to February, 2012. Participants were randomly allocated into one of the two interventions: 6 sessions of wet and dry cupping or heating pad application. The participants were offered an exercise program to perform during the participation period. A 0 to 100 numeric rating scale (NRS) for neck pain, measure yourself medical outcome profile 2 score (MYMOP2 score), cervical spine range of motion (C-spine ROM), neck disability index (NDI), the EuroQol health index (EQ-5D), short form stress response inventory (SRI-SF) and fatigue severity scale (FSS) were assessed at several points during a 7-week period. Results: Compared with a heating pad, cupping was more effective in improving pain (adjusted NRS difference: −1.29 [95% CI −1.61, −0.97] at 3 weeks ( p =0.025) and −1.16 [−1.48, −0.84] at 7 weeks ( p =0.005)), neck function (adjusted NDI difference: −0.79 [−1.11, −0.47] at 3 ( p =0.0039) and 7 weeks ( p <0.0001)) and discomfort (adjusted MYMOP2 difference score: −0.72 [−1.04 to −0.40] at 3 weeks and −0.92 [−1.24, −0.60] at 7 weeks). Significant improvement in EQ-5D was observed at 7 weeks (1.0 [0.88, 1.0] with cupping and 0.91 [0.86, 0.91] with heating pad treatment, p =0.0054). Four participants reported mild adverse events of cupping. Conclusion: Two weeks of cupping therapy and an exercise program may be effective in reducing pain and improving neck function in VDT workers.