The purpose of this study was to develop a procedure of depressive mood induction that operates the degree of the arousing mood by changing an instruction on situation image method. In study 1, 276 undergraduate students were asked to image 8 situations, and to rate degrees of depressed, anxiety, and positive mood in each situation. In the result, betrayal situation (a high depressive rousing situation) was selected as the situation that undergraduate students would arouse a depressive mood. In study 2, for operating the degree of the mood, instruction about the relationship, time and reason on the betrayal situation were changed. This 3 situations, betrayal situation (a high depressive rousing situation), and neutral situation were also rated depressive, anxiety, positive mood by 194 undergraduate students. In the result, the situation that changing a reason could only operate depressive mood, and maintain anxiety mood. As a result, this study found that arousing mood could be operated by changing the instruction of the situation on mood induction procedure. In the future, this mood induction procedure would contribute to the field of emotional investigation.