The purpose of this study is to develop the Two-Item Self-Esteem scale (TISE) and to investigate its reliability and validity. The TISE consists of two aspects of self-esteem concept: one is a self-evaluation, and the other is a self-acceptance. Concurrent validity was demonstrated by positive high correlations between the TISE and pre-existing self-esteem scales (i.e., Rosenberg's self-esteem scale). Construct validity was established by correlations between the TISE and criterion scales, including competence (i.e., narcissistic competence scale), subjective well-being (satisfaction with life scale), and positive/negative emotions (i.e., positive and negative affect schedule scales). The TISE and the Rosenberg's self-esteem scale had nearly identical correlations with these criterion scales. Test–retest reliability was confirmed at a both three-week and four-month interval. This study showed that the TISE is a reliable and valid scale to measure self-esteem with two aspects.