摘要：A total of 71 Staphylococcus aureus isolates associated with food-borne disease, healthy human nasal swabs, food samples, and healthy human feces were examined by PCR for the presence of classical staphylococcal enterotoxin (SE) genes ( sea, seb, sec, sed and see ), newly identified SE genes ( seg, seh and sei ), and methicillin-resistant S. aureus (MRSA)-related genes. Classical and newly identified SE genes were detected in isolates associated with food-borne disease, and some isolates associated with healthy human nasal swabs, food samples, and healthy human feces were shown to have the possibility of causing food-borne disease. Four isolates (three from healthy human nasal swab and one from healthy human feces) harbored the MRSA gene while three had the possibility of transmitting community-acquired MRSA. These results suggest that appropriate sanitary supervision is necessary to prevent contamination and infection with S. aureus . The PCR methods used in the study were useful for understanding the epidemiology and pathogenicity of S. aureus .
