A questionnaire survey was conducted in 2006, following previous surveys in 1990, 1996 and 2001, to ascertain the changing food preferences for seafood, meat and dairy products of 1342 children attending day nurseries in 17 Japanese cities. The proportions of children who liked seafood, meat and dairy products in the2006 survey were 73.1%, 73.6% and 77.0%, the figures for seafood and meat being no different among coastal, urban and inland dwellers. Although the proportion of children who liked seafood was not influenced by age, the proportion who liked meat increased with age. The children’s preferences for seafood and meat was influenced by their parents’ own preferences (p<0.01). The proportions of children who liked baked (87.3%) and raw seafood (62.8%) were higher than the figures in previous surveys, while the proportion of children who liked deep-fried seafood in 2006 (36.5%) was 15% lower than that in 1990. Of the 18 seafoods surveyed, the preferences were for shrimp (73.9%), salmon (73.7%) and tuna (68.1%). There was a stronger preference expressed in 2006 than that in previous surveys for 13 of the 18 seafoods. The results of the survey indicate that regional differences have decreased in the preferred seafoods and cooking methods.