The health-promoting effect of daily physical activity was assessed on walking courses recently established by a local government. We evaluated the quality and quantity of physical activity resulting from ten young females walking on three different courses located in Nagoya city. The length of each course was between8,600 and 10,000 steps. The exercise intensity level was estimated from the cardiovascular response (% HRR) and blood lactate levels, and the energy consumption was estimated from the count of a pedometer during walking. The results indicate that each course had potential health-promoting effects for middle- and old-aged individuals.