The sensitivity and the preference for five basic tasting substance were examined in DS strain mice. The mice were given various concentration of single and mixture of tasting substances dissolved in water, and examined as to drinking volume. On the other side, sensory evaluation was carried out in women students using the same materials. 1) The mice showed a high preference for sugars, followed by MSG and NaCl. Both citric acid and caffeine were not preferred. 2) Additional effects were seen strongly in the binary combinations, sucrose + saccharin and sucrose+ NaCl, and weakly in NaCl+MSG and citric acid+MSG. 3) The addition of a low concentration caffeine, citric acid or MSG singly to the mixture of sucrose and NaCl did not increase the intake volume over that of the original mixture. However, a combination of two of these substances caused an increase of intake, the mixture of the three substances was most preferred. 4) Women students preferred the single sucrose solution, and the more complicated the combination the greater the dislike.