We often use softening agents in washing. However, nobody knows how clothing treated with household softeners affects human senses. Five kinds of towels were treated with two different types of household softeners. After absorbing water, these samples were then used for sensory evaluation. It was learned that cotton and non-twist cotton towels treated with conventional softeners tended to cause cooler or wetter sensations than non-treated towels or towels treated with “improved” softeners. It is supposed that these differences were caused by differences of molecular structure of surfactants. Moreover, it is presumed that these differences were influenced by the combination of the softeners with the towel fibers, as well as by the structure of the towel fabrics. Thus, it is clear that dry and wet clothing treated with household softeners affects human senses.