Three groups of weanling male rats were fed for five weeks with diets, to which were added 0, 20 and 200mg/100g of roughly equal mixture of α-, β-, γ- andδ-tocopherols (Toc-0, Toc-20 and Toc200, respectively). After feeding, a comparative study on tissue uptake and accumulation of each tocopherol was made by analyzing tocopherols in various tissues of those rats, using high performance liquid chromatography. 1) No significant differences were found among three groups in growth of rat during feeding and in the relative organ weight after feeding. 2) In general, α-tocopherol showed the highest concentration of four forms in various tissues, and the tissue level of each tocopherol had a tendency to increase with increment in the diet. But, the extent of the tendency varied with different tissues and tocopherols; thus, that was the most manifest with each tocopherol level in small intestine, α-tocopherol level in liver and with δ-tocopherol level in adipose tissue. However, there was no significant difference in each tocopherol level in brain between Toc-20 and Toc-200 groups. 3) In Toc-20 group, showed nearly normal vitamin E level, no marked difference was noted in the concentration of α-tocopherol among various tissues except serum. In Toc-200 group, showed excessively high vitamin E level, it was found to accumulate preferentially α-tocopherol in liver, and δ- tocopherol in adipose tissue.