The detection of deterioration of photographic gelatin binders was examined from HPLC analysis of the sol fraction extracted into cold water from model gelatin films (alum-hardened, and non-hardened), RC paper, and baryta paper. It is confirmed that decomposed gelatins having relatively low molecular weight (-10⁴ Da or lower) was extracted and detected from all of the films after the accelerated ageing process. The detected amount increased with increasing the ageing time. We concluded that this technique is useful for the evaluation of degradation of gelatin binders.