Fibers seized from a crime scene are one of the most important evidence by providing useful information of the contact between the victim and the suspect, furthermore the suspect and the crime scene. A timeconsuming screening test is required before the following systematic identification. In the case of screening, the test for white fiber is more difficult, because the test includes visible color observation and morphological test of the cross section. Therefore, a fluorescent screening test of white fiber becomes extremely important for the forensic discrimination. In this paper, the new method for making the cross section is examined. First, about 5 mm length of single fiber is bonded to an adhesive tape (1 cm × 1 cm). Secondly, the tape is put on the side of a slide glass so that the fiber becomes vertical to the slide glass. Finally, the fiber on the adhesive tape is cut with a razor which could be used as a substitution blade. The obtained specimen can be used both for the observation of the cross section and for the fluorescent screening test. A fluorescent microscopy (340-380 nm) can be available for these purposes. This new method combining the observation of cross section and the measurement of fluorescence is quite useful for the forensic screening test of single fibers.