Turbulent channel flow is studied numerically by using Large-Eddy Simulation (LES). Finite-difference method is employed in the LES. The simulation is stably executed by using the 3rd order up-wind difference scheme which dissipate numerical errors. Several pilot tests are performed in order to investigate the numerical dissipation and the wall damping function. Time-dependent features and turbulent flow structures in a turbulent channel flow are numerically simulated. The agreement of the calculated mean velocity profile and the turbulence intensity with experimental data is good except near wall.