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  • 标题:木材腐朽菌の生化学的研究 (第II報) Bavendamm反応とtyrosinaseの関係について
  • 本地全文:下载
  • 作者:樋口 隆昌 ; 北村 和久
  • 期刊名称:日本森林学会誌
  • 印刷版ISSN:1349-8509
  • 电子版ISSN:1882-398X
  • 出版年度:1953
  • 卷号:35
  • 期号:11
  • 页码:350-354
  • 出版社:The Japanese Forest Society
  • 摘要:

    1) Employing the filtrate of cultured media, mycelial extract, and extracted mycelial residue of wood-rotting fungi (29 species), their laccase and tyrosinase activities were compared. Among these species, it was found that the tyrosine solution was oxidized by filtrate of cultured media of some species as follous: F. pinicola, L, saepiaria, P. Schweinitzii (brown rot fungi), F. fomentarius, Cortinellus edodes, Armillaria japonica (white rot fungi). 2) Inoculating the test fungi above mentioned on the nutrient agar cotaining 0.005M of anaphthol (for laccase test) and suitable amounts of tyrosine or p-cresol (for tyrosinase test), the tyrosinase reaction in addition to the laccase reaction were observed for some white rot fungi (Armillaria japonica, Cortinellus edodes, and F. f omentari us etc.). But for brown rot fungi, no laccase reaction was observed and tyrosinase reaction was observed for some species (F. pinicola, L. saepiaria, P. Schweinitzii, P. betulinus, Trametes albida). 3) From the above mentioned results, against LINDEBERG'S supposition, the extracellular tyrosinase was recognized for some species. Then, there are some probabilities that Bavendamm's reaction is caused by both laccase and tyrosinase for these species at least. However as a rule the content of tyrosinase and degree of secretion are little comparing with laccase. 4) Because tannic acid and gallic acid are oxidized with both laccase and tyrosinase and can not classify these enzyme reactions, it was proposed that employing z-naphthol for laccase test and p-cresol for tyrosinase test, and Bavendamm's reaction is divided into 2 test as stated above. 5) From the finding of LAW that the peroxidase was only observed in white rot fungi, some significance of peroxidase, related to lignin degradation, was supposed.

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