1) Transverse sections for microscopic study were prepared from two kinds of bamboo-shoot (Phyllostachys edulis and nigra var. Henonis) and phloroglucinol-HCI and Mäule reactions were tested. Both reactions were already positive around the spiral vessel at the apex of bambooshoot, then the reaction sphere was extended arround the vessel in vascular bundle along with older portion. But, parenchymatous tissue other than vascular bundle was negative, until the bamboo-shoot grew up about 5m. in hight. 2) Employing the section prepared above mentioned method, β-glucosidase and phenoloxidase reactions were tested by using p-nitrophenol-β-d-glucoside, p-phenilendiamine, hydroquinone and catechol as substrates, and these reactions were always positive around the same portion as lignin reaction appeared. 3) Some experiments were carried out for both β-glucosidase and phenoloxidase and following facts were cleared: a)β-glucosidase is alike to emulsin. b) phenoloxidase has a laccase nature. 4) The activity of these β-glucosidase and phenoloxidase was intense at the basal portion of bamboo-shoot and these facts suggested the relationship between lignif ication and these enzymes. 5) The determination of lignin and methoxyl content of lignin was made for the sample of each higher, middle and lower portion of bamboo-shoot. The methoxyl content was lower along with higher (younger) portion. 6) Based on the above mentioned facts, discussion for FREUbENBERG's lignin formation theory was made.