For breeding yeasts for plum wine with high ability of decomposing malic acid and with good flavor by protoplast fusion, selection of mutants were carried out from schizosaccharornyces pombe and Saccharomyces cereviciae. 554 mutants were obtained from S. pombe (p-1011) and S. cereviciae (Kyokai-1), from which 49 strains were selected by nystatin screening. These strains were incubated in plum juice for 3 days at 30°C for examining decomposition of malic acid, sensory test, fermentation rate and specific growth rate. Two strains were finally selected as candidates for protoplast fusion: uracil auxotroph E-1 from S. cereviciae, and arginine auxotroph C-6 from S. pombe.