Three black fungi multiplying in a brewery were identified. These were Cladosporium cladosporioides, Aureobacidium pullulans , and Aspergillus niger . The same numbers of Aspergillus oryzae and Aspergillus awamori were found as that of Cladosporium cladosporioides, Aureobacidium pullulans , and Aspergillus niger . The growth nutrients of these fungi were bolatile carbohydrates such as the ethanol and ester of brewing material and aerial N, and vapor condensations containing aerial dust and product of other fungi autolysis. Germicides such as Thiabendazol and 2-Methyltiazol were effective for a shortterm prevention of black fungi growth.