We conducted a survey for students about lifestyle habitats such as eating habits and monitored skin conditions to study the effects of the eating habit on skin conditions. This eating habit survey showed that n-6/n-3 ratio is on the increase due to the excessive consumption of fat and the insufficient intake of fish. Furthermore, there was a trend that there was a lack of many nutrients. For the relationship between nutrient uptake and skin conditions, there was a significant positive correlation between the take of n-6/n-3 and transepidermal water loss (TEWL), and the consumption of alachidonic acid and icosatrienoic acid and the extent of the incidence of acne. These results suggested that nutrient component taken from eating affects skin conditions. In addition, it is indicated that n-3 or n-6 fatty acid is strongly related to the barrier function of the horny cell layer and the incidence of acne.