Our objective is to investigate the apparent iron absorption rate between various phosphatidylcholines (PCs) and the type of dietary fatty acid. Young male rats were fed a diet containing eicosapentaenoic acid concentrate (EPA) or docosahexaenoic acid concentrate (DHA) for 3 weeks. These were then divided into two experiments. In one experiment when the rats were fed a diet containing 3% egg-yolk phosphatidylcholine (EggPC) with 2% olive, 2% EPA or 2% DHA. A significant difference, the was observed in the apparent absorption rate EggPC+EPA group was higher than the control. It was clarified that the apparent absorption rate was improved in the diet of the polyunsaturated fatty acids (PUFA) and EggPC. In another experiment, rats were fed a diet containing soybean phosphatidylcholine (SoyPC) similar to the first experiment. A significant difference, in the concentration of liver cholesterol was observed. SoyPC+DHA was lower than SoyPC+Olive. A significant difference in the apparent absorption rate, was observed SoyPC+EPA and SoyPC+DHA were lower than SoyPC+Olive. It was clarified that the apparent absorption rate decreased in the diet of PUFA and SoyPC. These results suggest that the apparent absorption rate is different based on the various PCs.