We have participated in a project that the Industrial Technology Center of Nagasaki advanced through collaboration between industry and academia and have tried to develop novel fermented biogenic foods for anti-aging and the prevention of cancer and liver disease using botanical lactic acid bacteria isolated from a product of the Nagasaki Prefecture. In this study, 620 isolated botanical lactic acid bacteria were used in the screening tests for selecting high-functional lactic acid bacteria. We obtained 219 fermented samples with an anti-oxidative activity, and 17 fermented samples were selected among them at random. As a result, 13 of the 17 fermented samples with the anti-oxidative activity suppressed the proliferation of HL60 cells and 14 fermented samples protected hepatocytes from necrosis induced by EtOH. Finally, 5 fermented samples were significantly positive in both tests. Novel foods for the maintenance and improvement of health will be developed using these high-functional botanical lactic acid bacteria in the future.