We studied the effects of the docosahexsaenoic acid concentrate (DHA) along with a high cholesterol (HC) diet given to rats on the epididymal fat pad weight, the serum and liver iron concentrations, and the apparent iron absorption rate. Young male rats were fed a diet containing 10% Butter, 10% DHA, 10% Butter+HC or 10% DHA+HC for 3 weeks. The epididymal fat pad weight decreased in the DHA group compared to the Butter group. The concentration of serum total cholesterol decreased in the DHA group compared to the Butter group, similar to the DHA+HC group when compared to the Butter+HC group. The concentration of serum iron increased in the DHA group compared to the Butter group and the concentration of liver iron decreased in the Butter+HC group and DHA+HC group compared to the Butter group and DHA group. It was clarified that the apparent absorption rates in the observed Butter+HC group and DHA+HC group were higher than the Butter group and DHA group fed the experimental diet for the 1-14 days. However the DHA group was lower than the Butter group fed the experimental diets for the 14-21 days. These results suggest that the concentration of total cholesterol decreased in the DHA+HC group compared to the Butter+HC group, and the accumulation of adipose tissue appeared to decrease in the DHA diet compared to the Butter group. From the apparent absorption rate for each week, it was clarified that the accumulation of iron was changed by the fatty acid composition of the sample lipids used in the experiment and different feeding periods. Furthermore, we are considering the iron intake by the body.