Nasal samples of humans and animals were examined for the presence of Staphylococcus aureus , and subsequently the incidence of enterotoxigenic and methicillin-resistant S. aureus. Additionally, characteristics of the isolates were studied. The carrier rate of S. aureus was 42.7% (38/89) for humans, 86.4% (57/66) for pigs, 19.8% (20/101) for cows, and 9.5%(4/42) for chickens. By using a commercial SET-RPLA kit, 22 isolates from humans produced staphylococcal enterotoxin (SE) A ( n =8), SEB ( n =9), and SEC ( n =5). S. aureus isolates were subjected to genotyping analysis for detection of se genes ( sea to see, seg, seh and sei ). The se genes were detected in 25 (65.8%) of 38 human, 34 (59.6%) of 57 pig, and 4 (100%) of 4 chicken isolates. Twenty-five human isolates possessed the sea ( n =8), seb ( n =4), seb-seg-sei ( n =5), sec-sei ( n =4), sec-seg-sei ( n =1) or seg-sei ( n =3) genes. Thirty-four isolates from pigs and 4 isolates from chickens possessed seg-sei genes. MRSA was detected from only 5 food handlers. Phenotypic and genotypic characteristics of 3 MRSA isolates from the 3 food handlers in the same facility were identical except for two phenotypic characteristics. Therefore, the results may suggest that the horizontal transmission of MRSA occurred in the facility.