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  • 标题:下痢症患者や鶏肉類から分離された Campylobacter jejuni のギランバレー症候群(GBS)関連遺伝子保有状況と薬剤耐性
  • 本地全文:下载
  • 作者:松田 正法 ; 徳島 智子 ; 重村 久美子
  • 期刊名称:日本食品微生物学会雑誌
  • 印刷版ISSN:1340-8267
  • 电子版ISSN:1882-5982
  • 出版年度:2013
  • 卷号:30
  • 期号:1
  • 页码:39-42
  • DOI:10.5803/jsfm.30.39
  • 出版社:Japanese Society of Food Microbiology
  • 摘要:

    To examine the relation of Campylobacter jejuni isolates to the development of Guillain–Barré syndrome, a total of 120 strains isolated from 56 gastroenteritis patients and 64 samples of chicken meat and giblet were characterized with serological test, PCR-detection of cst-II , cgtA , and cgtB genes associated with ganglioside-like mimicry of lipooligosaccharide (LOS), and antimicrobial susceptibility test. The 24 human-derived strains and the 19 chicken-derived strains were found to represent 12 and 10 different Penner's serogroups, respectively. Those three LOS genes were simultaneously detected from 9 human-derived strains and 9 chicken-derived strains. Among those 18 strains, only 1 chicken-derived strain carried cst-II (Asn51), and the other 17 strains carried cst-II (Thr51). The serogroups of the strains which harbored the three LOS genes were as follows: serogroup C (O : 3), O (O : 19) and R (O : 23, 36, 53), respectively in 3 human-derived strains; serogroup B (O : 2) in a chicken-derived strain; serogroup D (O : 4, 13, 16, 43, 50) in 4 chicken-derived strains. The antimicrobial sensitivity test showed a high frequency of resistance to 4 quinolones (nalidixic acid, norfloxacin, ofloxacin and ciprofloxacin) in 22 of 56 human-derived strains (39.3%) and 22 of 64 chicken-derived strains (34.4%). The frequency of tetracycline resistance was high: 24 of 56 human-derived strains (42.9%) and 16 of 64 chicken-derived strains (25.0%), however, that of fosfomycin resistance was low: 6 of 56 human-derived strains (10.7%) and 4 of 64 chicken-derived strains (6.3%). All 120 strains were sensitive to erythromycin.

  • 关键词:Campylobacter jejuni ; Guillain–Barré syndrome; lipooligosaccharide biosynthesis gene
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