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  • 标题:RAPD法を用いた国内産精米の品種判別技術
  • 本地全文:下载
  • 作者:大坪 研一 ; 藤井 剛 ; 橋野 陽一
  • 期刊名称:日本食品科学工学会誌
  • 印刷版ISSN:1341-027X
  • 电子版ISSN:1881-6681
  • 出版年度:1997
  • 卷号:44
  • 期号:5
  • 页码:386-390
  • DOI:10.3136/nskkk.44.386
  • 出版社:Japanese Society for Food Science and Technology
  • 摘要:

    As it is obliged that the cultivars, location and year of production should be displayed on the bag of milled rice grains, a powerful technique to identify them by objective methods is indispensable. Milled rice grains produced at the several agricultural experiment stations were subjected to the RAPD method. Each DNA was extracted by the CTAB method and amplified by the PCR method using 10 mer primers. It became possible to identify the cultivars by electrophoresis of the PCR products among the dominant 10 cultivars in Japan.

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