The purpose of this study was to examine the influence of work experience, education and location on work satisfaction in Micro, Small and Medium Enterprises (MSMEs) in Nigeria. A survey design of employees in 450 MSMEs across four states and business segments were used; 375 employees (83%) respondents were suitable for analysis. The Questionnaire measured demographics, location, education and experience as variables and data was analyzed using regression and correlation. The results suggest a significant positive relationship between educational qualifications, location and work satisfaction and negative correlation of previous experience and work satisfaction.