出版社：Japanese Society of Nutrition and Food Science
摘要：Actomyosin fraction extracted from muscle of octopus with Weber Edsall's solution was investigated. This protein fraction has an adenosintriphosphatase activity, which is activated by calcium, magnesium, manganese but inhibited by beryllium, zinc, fluorine, azide and parachloromercuricbenzoate. The protein fraction exhibits a remarkable viscosity drop in 0.6M KCl solution by the addition of 8×10-3M ATP, followed by the subsequent recovering of the reduced viscosity in the course of time. These facts suggest that in the muscle of octopus exists an actomyosin-like protein similar to the one of striated muscle of mammals. The crude extract with Weber-Edsall's solution shows a remarkable viscosity increase instead of viscosity drop to ATP and AMP at 20°C. The significance of these phenomena was not get clarified. Soluble ATPase which is soluble in dilute salt solution and precipitates in 35%saturated (NH4) 2 SO4solution was also found in the muscle of octopus, but this ATPase splits 2mols of phosphoric acid from ATP. The protein is supposed to be a single enzyme, apyrase or that contaminated by myokinase.
