A Survey for contamination of Salmonella from poultry farms in Shimane Prefecture was carried out during the period from April, 1993 to March, 1994. Salmonella were isolated from feces of laying hens and feed in 1 (7.7%) of 13 poultry farms and the detected serotype was only Salmonella Tenessee. The cause of contamination was bonito powder in feed. Salmonella had been isolated for 8 weeks after disinfection in a poultry farm contaminated by Salmonella , but no Salmonella were isolated there after.