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  • 标题:In Silico Comparison of the Hemicelluloses Xyloglucan and Glucuronoarabinoxylan in Protecting Cellulose from Degradation
  • 本地全文:下载
  • 作者:Indrakumar Vetharaniam ; Martin Upsdell
  • 期刊名称:Computation
  • 电子版ISSN:2079-3197
  • 出版年度:2015
  • 卷号:3
  • 期号:3
  • 页码:336-353
  • DOI:10.3390/computation3030336
  • 语种:English
  • 出版社:MDPI Publishing
  • 摘要:We used a previously developed simulation model of a plant cell wall and its enzymatic degradation to compare the abilities of two hemicelluloses, glucuronoarabinoxylan (GAX) and xyloglucan (XG), to protect cellulose microfibrils (CMFs) from attack by cellulose-degrading enzymes. Additionally, we investigated the effect of XG abundance on the degradation rate of CMFs in the presence of the same enzymes. Simulations were run using hypothetical cell-wall compositions in which the numbers and arrangement of CMFs and (1,3;1,4)-β-glucan were kept constant, but the proportions of GAX and XG were altered. Scenarios considered walls with low and equal proportions of either GAX or XG, and also low, medium and high proportions of XG in the absence of GAX. The rate of CMF degradation was much lower in walls with GAX than walls with XG, except for early in the simulation when the reverse held, suggesting that XGs were protecting CMFs by competitive inhibition. Increasing XG content reduced both the degradation rate of CMFs and the percent of XG degraded, indicating that activity of enzymes decreased with XG density despite XG being degradable. Glucose oligosaccharide breakdown products were analysed on the basis of the originating polysaccharide and their degree of polymerisation (DP). The presence of GAX as opposed to equal amounts of XG had some significant effects on the amount and profile of breakdown products from XG and CMFs.
  • 关键词:plant cell wall; lignocellulose; enzymatic degradation; enzymes; accessibility; competitive inhibition; rumen; second-generation biofuels, agent-based modelling; simulation model plant cell wall ; lignocellulose ; enzymatic degradation ; enzymes ; accessibility ; competitive inhibition ; rumen ; second-generation biofuels, agent-based modelling ; simulation model
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