期刊名称：Journal of Radiation Research and Applied Sciences
印刷版ISSN：1687-8507
出版年度：2015
卷号：8
期号：3
页码：446-458
DOI：10.1016/j.jrras.2015.03.008
出版社：Elsevier B.V.
摘要：Abstract Natural marine polysaccharides such as chitosan, sodium alginate and carrageenan; after depolymerization by different techniques, have shown promising plant growth promoting and other biological activities. Chitosan after irradiation with Co-60 gamma-rays have proven to be a miraculous plant growth promoter in different plants. Trigonelline; an anti-diabetic compound is obtained mainly from the seeds of Fenugreek (Trigonella foenum-graecum L.). Trigonelline counteracts diabetes mellitus (DM) through the operation of the mechanisms of insulin secretion, modulation of β-cell regeneration and stimulation of activity of glucose metabolism related enzymes. Considering the medicinal importance a pot experiment was conducted to investigate the effect of foliar application of Co-60 gamma irradiated chitosan (IC) with soil applied phosphorus supplement on growth, biochemical and quality attributes of fenugreek. Four concentrations of irradiated chitosan were used (0, 40, 80 and 120 mg L−1) individually as well as in combination with single dose of phosphorus 40 kg ha−1. Un-irradiated chitosan (UN) 40 mg L−1 and de-ionized water were used as control. {IC} significantly affected almost all the parameters including seed yield, trigonelline content and trigonelline yield. There were 10 treatments and among all, P40 + IC 40 mg L−1 proved to be the best, which increased total alkaloid content by 34.9%, seed yield by 125.4%, and trigonelline content by 17.8%. Contents of photosynthetic pigments and activity of nitrate reductase, carbonic anhydrase enzymes were also significantly enhanced.
关键词：Gamma irradiated chitosan; Phosphorus; Trigonella foenum-graecum; High Performance Liquid Chromatography; Trigonelline; Scanning Electron Microscope
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