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  • 标题:RNA Aptamers for Targeting Mitochondria Using a Mitochondria-Based SELEX Method
  • 本地全文:下载
  • 作者:Yuri Tawaraya ; Mamoru Hyodo ; Mst Naznin Ara
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:2014
  • 卷号:37
  • 期号:8
  • 页码:1411-1415
  • DOI:10.1248/bpb.b14-00112
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:

    The use of mitochondria-based systematic evolution of ligands by exponential enrichment (SELEX) was explored. Mitochondria were isolated from rat liver and confirmed intact by respiratory control index. Isolated mitochondria and a 2′-F RNA random library were mixed and the bound RNAs collected. The counter selection was applied with nucleus and unbound RNAs were collected. After 7 rounds of selection, two sequences (Mitomer1 and Mitomer2) were verified to bind to mitochondria and the truncated Mitomer2 (short Mitomer2) showed better binding to isolated mitochondria than Mitomer1.

  • 关键词:RNA aptamer; selection; mitochondria
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