This study examined the self-affirmation of university students, and identified the factors which have a significant and influential connection on their self-affirmation. For the purpose of this research, we employed the following terminology: “school life,” “the paternal relationship,” “the maternal relationship,” “the parents marital relationship,” and “gender distinction.” The sample consisted of 539 students, 285 males and 254 females in a teacher-training university. From exploratory factor analysis of the university students' self-affirmation, the results indicated seven factors which were named “nuisance,” “other people's evaluation,” “self-fulfillment,” “insistence,” “eagerness,” “personality,” and “anxiety.” “School life” had a significant influence on factors described as “nuisance,” “other people's evaluation,” “insistence,” “eagerness,” and “personality.” Furthermore, “the paternal relationship” had a significant influence on their self-affirmation. With regard to gender distinct results, in male students, “the parents' marital relationship” had a significant and positive influence on their self-affirmation. On the other hand, in female students, the negativity toward their parents had a significant and negative influence on their self-affirmation.