We examined the effects of live Lactobacillus paracasei NFRI 7415 isolated from Japanese fermented fish ( funa-sushi ) on the fecal cholesterol excretion of mice. Female BALB/c mice were fed a control diet ( CO ) and control diet + Lb. paracasei NFRI 7415 ( 10⁶ cfu/mL ) ( LB ) for 4 weeks. The plasma triacylglycerol and T-cholesterol concentrations of the LB group were lower than those of the CO group. Although no significant difference in the fecal bile acid concentration was observed in both groups, the fecal Tcholesterol excretion of the LB group increased during the experimental period. These results indicated that live Lb. paracasei NFRI 7415 has the capacity of fecal cholesterol excretion, and it effectively reduced the plasma T-cholesterol concentration.