Objective: To verify the effect of smoking prevention education for nursing students using the improved tobacco smoke collection method. Methods: The improved tobacco smoke collection method allows mainstream smoke and sidestream smoke from a cigarette to be separately extracted using a closed system. After collection, we performed gas measurement using a gas detection tube and the Schiff reagent method. We provided a lecture incorporating the experimental method for an experimental group (42 students), but only the lecture without the method for a control group (43 students). We surveyed the changes in The Kano Test for Social Dependence (KTSND) scores before and after the lecture and one month later. Results: In the experimental group, the total scores of the KTSND were 10.2±5.0 (mean±standard deviation) before the lecture, 5.8±4.1 after the lecture, and 6.9±4.8 one month later. On the other hand, the scores were 10.7±5.7, 7.5±5.8, and 9.7±5.5 in the control group before, after, and one month after the lecture, respectively. It is considered that the students understood “smoking is harmful to health” since this gas analysis method can be used to check for harmful gases visually. Conclusion: Result of this study suggest that this experimental method is useful for educating nursing students on the harmful effects of smoking.