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  • 标题:A successful strategy for the recovering of active P21, an insoluble recombinant protein of Trypanosoma cruzi
  • 本地全文:下载
  • 作者:Marlus Alves dos Santos ; Francesco Brugnera Teixeira ; Heline Hellen Teixeira Moreira
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2014
  • 卷号:4
  • DOI:10.1038/srep04259
  • 出版社:Springer Nature
  • 摘要:Structural studies of proteins normally require large quantities of pure material that can only be obtained through heterologous expression systems and recombinant technique. In these procedures, large amounts of expressed protein are often found in the insoluble fraction, making protein purification from the soluble fraction inefficient, laborious, and costly. Usually, protein refolding is avoided due to a lack of experimental assays that can validate correct folding and that can compare the conformational population to that of the soluble fraction. Herein, we propose a validation method using simple and rapid 1D 1H nuclear magnetic resonance (NMR) spectra that can efficiently compare protein samples, including individual information of the environment of each proton in the structure.
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