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  • 标题:Minimally destructive sampling of type specimens of Pyropia (Bangiales, Rhodophyta) recovers complete plastid and mitochondrial genomes
  • 本地全文:下载
  • 作者:Jeffery R. Hughey ; Paul W. Gabrielson ; Laurence Rohmer
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2014
  • 卷号:4
  • DOI:10.1038/srep05113
  • 出版社:Springer Nature
  • 摘要:Plant species, including algae and fungi, are based on type specimens to which the name of a taxon is permanently attached. Applying a scientific name to any specimen therefore requires demonstrating correspondence between the type and that specimen. Traditionally, identifications are based on morpho-anatomical characters, but recently systematists are using DNA sequence data. These studies are flawed if the DNA is isolated from misidentified modern specimens. We propose a genome-based solution. Using 4 × 4 mm2 of material from type specimens, we assembled 14 plastid and 15 mitochondrial genomes attributed to the red algae Pyropia perforata , Py. fucicola , and Py. kanakaensis . The chloroplast genomes were fairly conserved, but the mitochondrial genomes differed significantly among populations in content and length. Complete genomes are attainable from 19th and early 20th century type specimens; this validates the effort and cost of their curation as well as supports the practice of the type method.
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