期刊名称：Revista Internacional de Contaminación Ambiental
印刷版ISSN：0188-4999
出版年度：1999
卷号：15
期号：1
页码：49-53
语种：Spanish
出版社：Centro de Ciencias de la Atmósfera
摘要：Clomiphene citrate (CC), a drug employed as an ovulation inducer in patients with infertility problems, produced framshift mutations in the Ames test, only when it was exposed to aroelor 1254 induced S9 mixture. The use ofdifferent rat liver inducers permits a better comprehension of the metabolic pathways followed by CC in order to be activated to mutagenic metabolite. The results presented here show that rat liver homogenates induced with streptozotocine (STZ), which enhanced the levels ofCYF2EI, did not improved the mutagenicity ofCC previously seen for aroelor 1254 S9. The employrnent offenobarbital (FB) as a rat liver inducer gave similar results to those obtained with aroelor 1254. S9 induced with 3 methylcholanthrene (3MC), slightly enhanced the mutagenic effect ofCC on S. typhimurium TA98. These results suggest that a redox reaction mediated by CYFIA and probably CYF2B isozyrnes are responsible for the transformation ofCC to a mutagenic metabolite.
其他摘要：Clomiphene citrate (CC), a drug employed as an ovulation inducer in patients with infertility problems, produced framshift mutations in the Ames test, only when it was exposed to aroelor 1254 induced S9 mixture. The use ofdifferent rat liver inducers permits a better comprehension of the metabolic pathways followed by CC in order to be activated to mutagenic metabolite. The results presented here show that rat liver homogenates induced with streptozotocine (STZ), which enhanced the levels ofCYF2EI, did not improved the mutagenicity ofCC previously seen for aroelor 1254 S9. The employrnent offenobarbital (FB) as a rat liver inducer gave similar results to those obtained with aroelor 1254. S9 induced with 3 methylcholanthrene (3MC), slightly enhanced the mutagenic effect ofCC on S. typhimurium TA98. These results suggest that a redox reaction mediated by CYFIA and probably CYF2B isozyrnes are responsible for the transformation ofCC to a mutagenic metabolite.
